Changes of plasma membrane H<Superscript>+</Superscript>-ATPase activities of <Emphasis Type="Italic">Glycine max</Emphasis> seeds by PEG treatment

The soybean （Glycine max） Heihe No. 23 is sensitive to imbibitional chilling injury. Polyethylene glycol （PEG） treatment can improve chilling tolerance of soybean seeds to a certain extent. The changes of hydrolytic ATPase in plasma membranes and H^＋-pumping responses in soybean seeds were investigated during PEG treatments. Effects of exogenous calcium and exogenous ABA on the hydrolytic ATPase were also examined in order to understand the mechanism of chilling resistance. Highly purified plasma membranes were isolated by 6.0% aqueous two-phase partitioning from soybean seeds, as judged by the sensitivity of hydrolytic ATPase to sodium vanadate. PEG treatment resulted in a slight increase of the hydrolytic ATPase activity in 12 h. Then the activity decreased gradually, but still higher than the control. The H^＋-pumping activity increased steadily during PEG treatment. Exogenous calcium had both activating and inhibiting effects on the hydrolytic ATPase, but the activity was inhibited in soybean seeds treated with exogenous ABA. Results suggested that PEG treatment, not the exogenous calcium and ABA, up-regulated H^＋-ATPase activities in soybean seeds.     

关于等离子体种子处理技术的田间试验研究

本文阐述了等离子体种子处理技术机理,设计了田间试验示范操作规程,分析了试验工作特点,评述了实际成效,阐明了技术推广前景。     

我国粮食仓储技术的概况与发展

粮食安全储存是个世界性难题,如何发展、提升和完善粮食仓储技术是我国政府面临的一项亟待解决的课题.为此,在提出粮食仓储技术内涵的基础上,首先概述总结了我国粮食仓储设施及方式的发展历程;其次在分析国内储粮技术研究发展现状的基础上,重点介绍了低温储粮、气调储粮两种绿色环保型储粮技术及计算机技术在仓储上的应用;论述了我国仓储设备的整体发展现状;最后指出了我国仓储技术的存在的问题与发展趋势.     

基于高压电技术的烟气脱硫研究与应用进展

分析与评述了国内外基于高压静电技术的烟气脱硫研究与应用进展,介绍了几种以高压电技术为基础的烟气脱硫新技术--电子束烟气脱硫、脉冲电晕等离子法脱硫、荷电干法烟气脱硫和荷电喷雾烟气脱硫技术;并且分析了这几种烟气处理技术的作用原理、特点和发展现状,展望了其应用前景.     

机动车排放净化技术研究

机动车排放污染严重，本文简要介绍了净化机动车排放的方法．探讨了低温等离子体净化尾气的反应机理，阐述了两种低温等离子体发生器。     

电控发动机的氧传感器波形分析

通过汽车示波器对氧传感器的信号电压波形测试,介绍了氧传感器的标准信号电压波形、氧传感器波形分析法诊断发动机故障的一般流程及其具体应用。     

基于遗传算法与RBF网络的养殖池塘溶解氧模型

在分析了工厂化水产养殖池塘溶解氧影响因素的基础上,利用RBF神经网络良好的非线性逼近能力建立了池塘溶解氧的神经网络预测模型.常规的RBF神经网络模型常导致训练时间较长且易陷入局部极小点,因此,采用自适应遗传算法对RBF神经网络进行优化,模型的收敛速度明显加快.采用了养殖池塘的外部可控环境水体温度T、水流量Q、酸度(pH)以及增氧机器的转速V作为模型的输入.实验结果表明采用该方法预报溶解氧的预测精度较常规RBF递推算法的预测精度明显提高.该方法为研制开发智能水产养殖环境监控系统以及工厂化水产养殖奠定了基础.     

浅谈汽车氧传感器的检修

氧传感器是现代轿车上应甩广泛的传感器之一,对发动机的正常工作和尾气排放控制起着至关重要的作用.文章叙述氧传感器的工作原理,由氧传感器引发的故障实例,介绍检测与维修方法.     

工厂化水产养殖智能监控系统设计

提出了一种工厂化水产养殖中具有可溯源功能的智能监控系统.系统利用PROFIBUS-DP总线和工业以太网技术实现了数据的传输与共享,利用模糊控制与神经网络相结合的算法实现了对数据的处理分析,并得到控制信号,进行闭环控制,利用无线射频识别技术( RFID)实现了水产品质量的可溯源功能.结果表明,养殖环境各关键环境因子均满足控制精度,水产品的可溯源信息写入与读出均完整有效,完全达到了设计要求,能够满足工厂化水产养殖智能化的需要.     

Protective effect of BNDF on vascular endothelial cells with H2O2-induced oxidative injury

AIM: To study the protective effect of brain-derived neurotrophic factor (BDNF) on vascular endothelial cells with H₂O₂-induced oxidative injury. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro, and the oxidation injury model of HUVECs was established by treatment with H₂O₂. The oxidatively injured HUVECs were cultured with different concentrations (1, 10 and 100 μg/L) of BDNF. At the same time, the control group (no injury), PBS treatment after H₂O₂ injury group and TrkB inhibitor group (with 100 μg/L BDNF and 1: 1 000 TrkB inhibitor) were also set up. The viability of the HUVECs was detected by MTT assay. The levels of LDH, MDA, SOD and GSH were measured. The releases of NO, ET-1 and ICAM-1 were analyzed by ELISA. The changes of ROS production and cell apoptosis were evaluated by flow cytometry. The protein levels of TrkB, p-TrkB, cleaved caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with uninjured control group, in H₂O₂ oxidative injury plus PBS treatment group, the viability of the cells was decreased significantly, the LDH and MDA levels were increased significantly and the activities of SOD and GSH were decreased significantly. The NO secretion was decreased, and the ET-1 and ICAM-1 concentrations were increased significantly. The ROS content and apoptotic rate were increased significantly. The protein levels of cleaved caspase-3 and Bax were increased but Bcl-2 protein expression was decreased significantly. Compared with PBS treatment group, in H₂O₂-injured HUVECs treated with different concentrations of BDNF, the cell viability was gradually increased, the LDH and MDA levels were decreased and the activities of SOD and GSH were increased gradually. The secretion of NO was increased but ET-1 and ICAM-1 were decreased gradually. The ROS content and apoptotic rate were decreased significantly. The TrkB and p-TrkB levels were significantly increased significantly, the protein expression of cleaved-caspase 3 and Bax was decreased gradually and the Bcl-2 protein expression increased gradually. The role of BDNF was inhibited by TrkB inhibitor. CONCLUSION: BDNF protects HUVECs from oxidative injury by binding with TrkB to activate the BDNF-TrkB signaling pathways.